Authors: Tran Van Hai, Nguyen Thi Hong Ngoc, Tran Thanh Duong, Nguyen Thi Huong Binh, Nguyen Thu Huong
Fasciola infestation in humans is known to have species Fasciola gigantica and Fasciola hepatica. These species also cause diseases mainly of ungulates such as buffaloes, cows, and sheep.. The disease has also been reported in 75 countries around the world. The diagnosis of disease in humans and animals is mainly based on egg and fluke morphology, or immunology and clinical symptoms. These techniques are often late and confusing, while molecular biology is costly, especially in undeveloped countries like Vietnam. Developing a fast and accurate detection technique is very important and necessary. In this study, we developed the LAMP technique to quickly and accurately detect Fasciola spp. from different kinds of samples such as feces, tissue human samples with a sensitivity and specificity of over 95%. This method will help increase sensitivity, reduce engineering time, and lower cost, this is considered a superior advantage over other diagnostic techniques. The primer set was designed for founding on ITS1-2 gene. A Positive reaction was visualized with the naked eye when using the color indicator, Malachite Green.
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