Authors: Shoshi Inooka.
DNA crown cells (artificial cells), in which the outside of the membrane is covered with DNA, can be synthesized in vitro using sphingosine (Sph)-DNA-adenosine-monolaurin compounds. These cells can proliferate within egg whites in vivo. In a previous report on the culture and synthesis of synthetic DNA crown cells, it was shown that different lines of synthetic DNA crown cells (i.e., E. coli, Human placenta, Akoya pearl oyster, Hepato-blastomaderived cell line; HepG2) could be prepared using egg white as a medium.
The present study examined whether the cell assembly and proliferation were observed when synthetic DNA (E. coli) crown cells were cultured on agar plates with monolaurin. In addition, the microscopic characteristics of the assemblies and the proliferated cells are described.
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