Food Science & Nutrition Research

Open Access ISSN: 2641-4295

Abstract


In Vitro and In Vivo Antioxidant Properties of P-Coumaroyl Alphitolic Acid Extracted from Fruit of Zizuphus mauritiana

Authors: Doumta Charles Falang, Biyanzi Pierre

Fruit of Zizuphus mauritiana has been commonly used in Traditional Medicine for its various pharmacological activities, such as its anticancer, antiepileptic, anti-inflammatory, anti-insomnia, neuroprotective effects and antioxidants. The aime of this study is to extract p-coumaroyl alphitolic acid compound present in fruit of Z. Mauritiana and to evaluate the antioxidant potential both in In-vitro and In-vivo effects. In-vitro antioxydant activities were assessed by DPPH, ABTS and FRAP methods. In-vivo antioxydant effects were evaluated in rats for 14 days, where different concentrations of p-coumaroyl alphitolic acid extracts (ACAE) (50, 100, 150, and 200 mg/kg/day) were orally administrated daily. Antioxidant profiles were performed by measuring malondialdehyde (MDA), blood proteins, reduced glutathione (GSH), glutathione peroxydase (GPX), catalase (CAT) and superoxide dismutase (SOD) activities in red blood cells. ACAE revealed excellent free radical scavenging activity (IC50) with DPPH (7.29 ± 0.94 µg/ml), ABTS (2.84 ± 0.07 µg/ml) and FRAP (28.32 ± 0.66 µg/ml) assay. Furthermore, CMAE decreased the level of MDA and SOD, and increased levels of GSH, GPX and CAT in blood. The results showed good antioxidant properties of ACAE of Zizuphus mauritiana fruit. Base on this results Jujube might be useful for the treatment of oxidative-stress related diseases such as chronic deseases.

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