Authors: Modesto Millán, Jorge Miranda, Lourdes Rodríguez-Fragoso, Jorge Reyes-Esparza
Pediococcus pentosaceus is a probiotic-bacterium able to confer a health benefit on the host because this bacterium can prevent the pathogen adhesion to intestinal cells as well as could tolerate bile salts, gastric and intestinal conditions. In particular, there are evidence that P. pentosaceus attenuates the severity of colitis and improve the intestinal permeability. By this reason is important stablishing the adequate conditions for the growth of probioticbacterium P. pentosaceus. The aim of the present study was evaluated the effect of temperature in shaken flasks, as well as the effect of pH and stirring speed on the growth and cell viability of P. pentosaceus using a 22 factorial design. We found that in the range of 34 to 40°C, the temperature affected the specific growth rate, biomass production and cell viability of probiotic-bacterium P. pentosaceus, being 37°C the optimum temperature condition for the culture of the bacterium. In bioreactor cultures, we observed that the stirring speed and pH not affected the biomass production and cell viability. The specific growth rate only was affected by the pH and we obtained the equation: µ = 0.95 + 0.064 pH with an R-squared value of 0.964. These findings allow establishing the conditions of suitable cultures to advantage the growth of probiotic-bacterium P. pentosaceus increasing biomass production and their cell viability.
View/Download pdf